Product concept and prototype flexibility in manufacturing: Implications for customer satisfaction

A rapidly changing competitive landscape and dynamic customer expectations require manufacturing firms to seek flexibility in product development. Product concept flexibility (i.e., developing design options) and product prototype flexibility (i.e., creating working models) emerge as effective ways to quickly develop new products that meet competitive challenges and satisfy customer demands. Product concept flexibility enables firms to fully explore various product definitions and ideas. Product prototype flexibility allows firms to gather customers’ feedback and investigate design feasibility. Using data from 273 manufacturing firms, this research tests mediating, moderating, and additive models that relate product concept flexibility, product prototype flexibility, and customer satisfaction. The results indicate that firms with high product concept flexibility are more likely to benefit from prototype flexibility than firms with low product concept flexibility, and that product concept flexibility and product prototype flexibility act independently and additively to predict customer satisfaction.     

A PRECISE INTEGRATION APPROACH FOR THE DYNAMIC-STIFFNESS MATRIX OF STRIP FOOTINGS ON A LAYERED MEDIUM 1）

提出应用精细积分算法计算多层地基的动力刚度问题.精细积分是计算层状介质中波传播的高效而精确的数值方法.利用傅里叶积分变换将层状地基的波动方程转换为频率-波数域内的两点边值问题的常微分方程组,运用精细积分方法求解格林函数,最后再将得到的频率-波数域内地基表面的动力刚度矩阵转换到频率-空间域内,进而得到刚性条带基础频率域的动力柔度或刚度矩阵.所建议的精细积分算法,可以避免一般传递矩阵计算中的指数溢出问题,对各种情况有广泛的适应性,计算稳定,在高频段可以保障收敛性,并能达到较高的计算精度.     

分子动力学模拟极端嗜热核糖结合蛋白的热力学稳定性

采用分子动力学模拟方法研究极端嗜热性核糖结合蛋白(tteRBP)的嗜热机理.在常温(300 K)和最佳活性温度(375 K)时,分别对tteRBP分子进行动力学模拟,结果表明,整体分子均保持结构稳定,但分子内部的协调运动不同.在375 K时蛋白整体柔性显著提高,使分子能够局部调整构象以适应极端高温.蛋白结构变化的分析也确认了高温时构象局部微调对蛋白极端高温稳定性的关键作用.     

The influence of composition and structure of water-in-oil microemulsions on activities of Iraqi Turnip peroxidase

The activity of the enzyme Iraqi Turnip peroxidase (ITP) is studied in a reverse microemulsion composed of chloroform, aqueous buffer, sodium dodecylsulfate (SDS) and alcohols of the homologous series 1-propanol to 1-hexanol through the measurements of absorbancy of the product of oxidation at the wavelength of 470 nm in the course of reactions. The ITP catalyzed reaction is the oxidation of guaiacol by hydrogen peroxide. Maximum enzyme activity was obtained at ω₀ (molar ratio of water to surfactant) = 8. It was found that the oxidation reaction obeyed Michaelis–Menten kinetics in the investigated concentration rang (0.08–0.8 mM) of the substrate, and the Michaelis constant K_m and maximal reaction rate V_m were determined. The enzyme inhibition caused by the alcohols in microemulsions is a consequence of both the solubility of the alcohols in the buffer and the flexibility of the interfacial film.     

Control methods for dynamic time-based manufacturing under customized product lead times

For manufacturers, the integration of high performance manufacturing with customer-oriented practices plays an important role in improving the performance of their business system. The benefits from such integration can only be maximized when the two parts are designed to work cooperatively. Though previous research has contributed much to manufacturing control algorithms and customer service practices, there has been little consideration of the two parts as a whole; consequently, the methods proposed may not be well supported by the other practices adopted in the system. This study develops production control methods that support a customer-oriented lead time policy, and aims to increase the performance of both manufacturing and customer service. The control methods are proposed for hybrid flow shops handling orders arriving dynamically. Computer simulations are conducted on a large number of problem instances, and the results show that the designed distributed feedback and decision-making functions enable the proposed methods to significantly outperform existing methods in achieving just-in-time (JIT) job completion under customized product lead times. Even taking into account the possible tradeoff between JIT job completion and flow time length, the proposed methods still deliver competitive performance.     

Modeling loop backbone flexibility in receptor‐ligand docking simulations

The relevance of receptor conformational change during ligand binding is well documented for many pharmaceutically relevant receptors, but is still not fully accounted for in in silico docking methods. While there has been significant progress in treatment of receptor side chain flexibility sampling of backbone flexibility remains challenging because the conformational space expands dramatically and the scoring function must balance protein–protein and protein–ligand contributions. Here, we investigate an efficient multistage backbone reconstruction algorithm for large loop regions in the receptor and demonstrate that treatment of backbone receptor flexibility significantly improves binding mode prediction starting from apo structures and in cross docking simulations. For three different kinase receptors in which large flexible loops reconstruct upon ligand binding, we demonstrate that treatment of backbone flexibility results in accurate models of the complexes in simulations starting from the apo structure. At the example of the DFG‐motif in the p38 kinase, we also show how loop reconstruction can be used to model allosteric binding. Our approach thus paves the way to treat the complex process of receptor reconstruction upon ligand binding in docking simulations and may help to design new ligands with high specificity by exploitation of allosteric mechanisms. © 2012 Wiley Periodicals, Inc.     

多跨梁离散系统的频谱和模态的定性性质

采用差分法建立了两端任意支承多跨梁的差分离散模型,对只具有两端支承的单跨梁是正系统的情形,导出了对应的多跨梁差分离散系统的柔度系数;从单跨梁正系统的刚度矩阵的符号振荡性出发,通过引入辅助系统,讨论了多跨梁的柔度矩阵的振荡性,确定了对应于单跨梁正系统的多跨梁差分离散系统的频谱和模态的一系列定性性质.      

THE STRESS SUBSPACE OF HYBRID STRESS ELEMENT AND THE DIAGONALIZATION METHOD FOR FLEXIBILITY MATRIX H

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Small molecule diffusion in a rubbery polymer near Tg: Effects of probe size,shape, and flexibility

A novel experimental approach involving fluorescence nonradiative energy transfer (NRET) is employed to study the Fickian diffusion of small molecules in rubbery poly(isobutyl methacrylate) (PiBMA) films near the glass transition, using a formalism that directly relates the small molecule translational diffusion coefficient, D, to changes in the normalized nonradiative energy transfer efficiency, E_N. Values of D for pyrene, 1,3-bis-(1-pyrene) propane (BPP), 1,3-bis-(1-pyrene) decane (BPD), 9,10-bis-phenyl ethynyl anthracene (BPEA), diphenyl Disperse Red 4 (DPDR4), and decacyclene in PiBMA are measured over temperatures ranging from approximately T_g to T_g + 25°C. Among these chromophores, significant differences in both the magnitude and temperature dependence of D are observed which are attributed to differences in molecule shape and flexibility, as well as molar volume. Other factors being equal, chromophore flexibility was shown both to increase the magnitude of D and to decrease its dependence on temperature, as does an increase in aspect ratio. For BPD, these effects are attributed to the ability of the flexible molecule to diffuse in a piecewise manner, requiring the cooperative mobility of fewer polymer chain segments than a rigid molecule of the same molar volume. For BPEA and DPDR4, this deviation from D being dominated by molar volume effects is attributed the to high aspect ratio of these elongated molecules. © 1996 John Wiley & Sons, Inc.     

Teacher flexibility in mathematical discussion

The significance of discussion in mathematics classes has been prominently debated in the research literature. Different studies have stressed the importance of teacher flexibility in orchestrating the discussion. We introduce an operational definition of teacher flexibility. In a case study with one secondary-school mathematics teacher, we microanalyzed discussion situations in which the teacher had to change her plan according to unforeseen student replies. The analysis was aimed at characterizing situations in which the teacher was either flexible or inflexible in her interactions with students and describing the factors that affected her flexibility. We suggest four basic patterns of teacher flexibility and discuss the complexity of the factors that shape them.